Why do we need to standardize DCPIP?

Why do we need to standardize DCPIP?

There are several oxidising agents that can be used and a commonly used one is 2,6-dichlorophenol-indophenol or DCPIP. You need to standardise this against a known concentration of vitamin C. This means finding out how much DCPIP reacts with a known amount of vitamin C. How do they compare to DCPIP?

What is DCPIP used to test the presence of?

2,6-dichlorophenolindophenol (DCPIP) can be used to estimate the concentration of vitamin C in food. DCPIP is blue when dissolved in water, is red in acid conditions, and is reduced by ascorbic acid (vitamin C) to a colourless compound.

How does DCPIP test for vitamin C?

Ascorbic acid reacts with DCPIP, changing the colour from blue to colourless. They react in a 1:1 fashion, so if a known quantity of DCPIP solution reacts with the plant tissue extract, the quantity of DCPIP used gives a direct measure of the quantity of ascorbic acid present.

What is Indophenol used for?

Indophenol is used in hair dyes, lubricants, redox materials, liquid crystal displays, fuel cells and chemical-mechanical polishing. It is an environmental pollutant and is toxic to fish.

Which fruit has the most vitamin C?

Fruits with the highest sources of vitamin C include:

• Cantaloupe.
• Citrus fruits and juices, such as orange and grapefruit.
• Kiwi fruit.
• Mango.
• Papaya.
• Pineapple.
• Strawberries, raspberries, blueberries, and cranberries.
• Watermelon.

What is the function of DPIP in this experiment?

The function of the DPIP in this experiment is to act as the electron acceptor, replacing the usual NADP found in plants. When the light shines on the active chloroplasts, the electrons are excited, which causes them to jump to a higher energy level thus reducing the DPIP.

What is the titration that used for vitamin C?

One way to determine the amount of vitamin C in food is to use a redox titration. The redox reaction is better than an acid-base titration since there are additional acids in a juice, but few of them interfere with the oxidation of ascorbic acid by iodine.

Is indophenol toxic?

Irritating to eyes, respiratory system and skin. The material has NOT been classified as “harmful by ingestion”. The material may still be damaging to the health of the individual, following ingestion, especially where pre-existing organ (e.g. liver, kidney) damage is evident.

What is Nesslerization method?

Brief Method Summary. A sample is buffered at alkaline pH with borate buffer to decrease hydrolysis of cyanates and organic nitrogen compounds, and is distilled into a solution of boric acid. The ammonia distillate is determined colorimetrically by Nesslerization, or by other options given in the method.

How long does vitamin C stay in the body?

As a water-soluble vitamin, vitamin C does not remain in your body very long. In most cases, this essential nutrient is excreted within 24 hours, but the amount of time vitamin C stays in your system depends on your individual circumstances and medical history.

What is iodometric titration?

Iodometry, known as iodometric titration, is a method of volumetric chemical analysis, a redox titration where the appearance or disappearance of elementary iodine indicates the end point. The iodometric titration is a general method to determine the concentration of an oxidising agent in solution.

How do you find the amount of DCPIP reduced?

The amount of DCPIP reduced can be found by measuring the solution’s light transmittance with a spectrophotometer. DCPIP can also be used as an indicator for Vitamin C. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid.

What is the function of dcdpip in photosynthesis?

DCPIP is a redox dye commonly used as a monitor of the light reactions in photosynthesis because it is an electron acceptor that is blue when oxidized and colorless when reduced. It is part of the Hill reagents family. DCPIP is commonly used as a substitute for NADP+.

What happens to DCPIP when light is added to a solution?

When DCPIP is present, it also gets reduced by the light reaction. The amount of DCPIP reduced can be found by measuring the solution’s light transmittance with a spectrophotometer. DCPIP can also be used as an indicator for Vitamin C.

What is DCIP titration?

2,6-Dichloroindophenol (DCIP) titration is an established method to determine l -AA content. DCIP works on the principle of l -AA reduction to a colorless solution from the deep blue color of the oxidized dye.