What is Bisalbuminemia?
Bisalbuminemia or alloalbuminemia is an inherited or acquired, rarely encountered serum protein anomaly, characterized by the occurrence of bicuspid electrophoretic pattern in the albumin fraction detected on serum electrophoresis. It can be seen in densitometry as a bifid mountain where albumin has two heads.
What is protein electrophoretic fractionation?
In electrophoresis proteins are fractionated according to their rate of movement in an electrical field; this is dependent on the charge on the protein molecule and on its size and shape. In this procedure serum proteins are divided into the principal fractions: albumin; alpha, beta and gamma globulins, and fibrinogen.
What buffer is used in serum electrophoresis?
In DNA electrophoresis, buffers like TAE (Tris-acetate-EDTA) and TBE (Tris-borate-EDTA) are used most commonly. In protein electrophoresis, SDS (sodium dodecyl sulfate) is commonly used. These buffers facilitate the separation of the samples into readable gels.
Why protein electrophoresis is done?
The test separates proteins in the blood based on their electrical charge. The protein electrophoresis test is often used to find abnormal substances called M proteins. The presence of M proteins can be a sign of a type of cancer called myeloma, or multiple myeloma.
Why electrophoresis test is done?
Hemoglobin electrophoresis measures hemoglobin levels and looks for abnormal types of hemoglobin. It’s most often used to help diagnose anemia, sickle cell disease, and other hemoglobin disorders.
How is protein electrophoresis done?
It involves exposing serum placed in a special type of gel to an electric current. This causes the different types of proteins to move and group together. The proteins create separate bands on the gel, which are then analyzed by the laboratory.
What can electrophoresis diagnose?
What is M band in electrophoresis?
A dense narrow band that is composed of a single class of immunoglobulins secreted by an abnormally expanded clone of plasma cells is known as M-protein (paraprotein, monoclonal protein, or M-component). Monoclonal pattern serum protein electrophoresis (SPEP).
What is M band?
Usually the C-reactive protein band is found between the beta and gamma regions. Serum protein electrophoresis is most commonly ordered when multiple myeloma is suspected and observation of a monoclonal band (M band, paraprotein) indicates that monoclonal gammopathy may be present in the patient.
What is the normal range of protein electrophoresis?
6.4 to 8.3 grams per deciliter
Normal Results Normal value ranges are: Total protein: 6.4 to 8.3 grams per deciliter (g/dL) or 64 to 83 grams per liter (g/L) Albumin: 3.5 to 5.0 g/dL or 35 to 50 g/L. Alpha-1 globulin: 0.1 to 0.3 g/dL or 1 to 3 g/L.
Do you need to fast for serum protein electrophoresis?
No preparation is needed for the test. When you arrive, a healthcare professional will simply use a needle to take a blood sample. Some people experience mild pain when the needle is inserted. There may be some slight bruising afterward.