What does peptone Iron Agar test for?

What does peptone Iron Agar test for?

Peptone Iron Agar is used for detecting hydrogen sulfide production by microorganisms. Levine and co-workers1,2 described a medium containing proteose peptone and ferric citrate for detection of hydrogen sulfide production by coliform bacteria.

How does peptone Iron Agar detect the production of hydrogen sulfide gas?

Peptone Iron Agar scores over Lead Acetate Agar, a medium to detect H2S, in giving clear and early results (4). Sodium thiosulphate and ferric ammonium citrate form the H2S detecting system. Sulphide is released from thiosulphate due to the action of bacterial enzymes.

What is the purpose of kligler iron agar test?

Kligler Iron Agar is used for the differentiation of microorganisms in the Enterobacteriaceae family on the basis of dextrose and lactose fermentation and hydrogen sulfide production.

What is the purpose of Kia test?

Kliger’s Iron Agar (KIA) This is a differential medium. It tests for organisms’ abilities to ferment glucose and lactose to acid and acid plus gas end products. It also allows for identification of sulfur reducers.

What does a negative result indicate Kia?

What indicates a Negative result? No fermentation occurred.

What is TSI test in microbiology?

The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism’s ability to ferment sugars and to produce hydrogen sulfide. It is often used to differentiate enteric bacteria including Salmonella and Shigella.

What does a positive Kia test look like?

Positive Test – Bubbles in the medium, cracking and displacement of the medium, or separation of the medium from the side and bottom of the tube. Negative Test – No bubbles and no separation or displacement of the medium.

What is the difference between KIA and TSI?

TSI is similar to Kligler’s iron agar (KIA), except that Kligler’s iron agar contains only two carbohydrates: glucose (0.1%) and lactose (1%). 0.1% glucose: If only glucose is fermented, only enough acid is produced to turn the butt yellow. The slant will remain red.

What is the result of Kia for Escherichia coli?

Result interpretation of triple sugar iron test on KIA A yellow butt (acid production) and red pink slope indicate the fermentation of glucose only. A yellow slope and a yellow butt indicate the fermentation of lactose and possibly glucose. This occurs with E. coli and other enterobacteria.

What is the PH indicator in Kia?

Phenol red
Phenol red is an indicator of carbohydrate fermentation. Fermentation reactions are read on the slant and in the butt, indicated by a color change from red (alkaline) to yellow (acid). The dextrose concentration in KIA is one-tenth the concentration of lactose.

How do you read TSI results?

Expected results of TSI Agar test are:

1. Alkaline slant/no change in butt (K/NC) i.e Red/Red = glucose, lactose and sucrose non-fermenter.
2. Alkaline slant/Alkaline butt (K/K) i.e Red/Red = glucose, lactose and sucrose non-fermenter.

What is the principle of TSI test?

Principles of the Procedure TSI Agar contains three sugars (dextrose, lactose and sucrose), phenol red for detecting carbohydrate fermentation and ferrous ammonium sulfate for detection of hydrogen sulfide production (indicated by blackening in the butt of the tube).

What is peptone iron agar used for?

Peptone Iron Agar is used for detecting hydrogen sulfide production by microorganisms. Summary and Explanation Levine and co-workers1,2described a medium containing proteose peptone and ferric citrate for detection of hydrogen sulfide production by coliform bacteria.

What is the composition of iron agar?

Kligler Iron Agar, in addition to peptone, HM peptone B and yeast extract, contains lactose and glucose (dextrose), which enables the differentiation of species of enteric bacilli. Phenol red is the pH indicator, which exhibits a colour change in response to acid produced during the fermentation of sugars.

Why do we use Kligler Iron Agar in microbiology?

To differentiate organisms by demonstrating hydrogen sulfide production and the fermentation of dextrose and lactose. Kligler Iron Agar, in addition to peptone, HM peptone B and yeast extract, contains lactose and glucose (dextrose), which enables the differentiation of species of enteric bacilli.

How to determine hydrogen sulphide in enteric Gram-negative bacilli?

It is also recommended that determination of the Hydrogen sulphide depends on the production of the Enteric gram-negative bacilli and also in detecting the production of H2S by using some strains of Pseudomonas. What is Kligler Iron Agar Test? Kligler iron agar test is used for detecting the fermentation of carbohydrate in the medium.