What are the markers for dendritic cells?
In humans, all DCs express high levels of MHC class II (HLA-DR) and lack typical lineage markers CD3 (T cell), CD19/20 (B cell) and CD56 (natural killer cell).
What is CD11c marker for?
CD11c, also known as integrin alpha X, is the most widely used defining marker for dendritic cells (DCs). CD11c can bind complement iC3b and mediate phagocytosis in vitro, for which it is also referred to as complement receptor 4.
What are CD11c+ cells?
In T1D, islet CD11c+ cells are a mix of resident and recruited macrophages, dendritic cells (DCs), and inflammatory monocytes (4, 9, 10). Depletion of CD11c+ cells or removal of the pancreatic draining lymph node (PLN) prior to islet infiltration, prevents T1D progression in non-obese diabetic (NOD) mice (11–13).
Are dendritic cells CD11b+?
Dendritic Cells in Nonlymphoid Tissues cDCs represent 1–5% of tissue cells depending on the organ and consist of two major subsets: CD103+CD11b− and CD11b+ cDCs.
What is the difference between pDC and cDC?
A series of studies have outlined distinct differences in the ability of DC subsets to induce immune responses. In particular, cDC have been implicated in driving a pro-allergic response, while pDC have been identified to block or “tolerize” the pulmonary immune environment against Th2 responses .
How do you identify dendritic cells?
Dendritic Cells markers To date, no single cell marker has been found to be expressed exclusively on DCs, therefore a combination of the presence and absence of various cell markers can be used to identify the DCs.
Do all dendritic cells express CD11c?
However, CD11c is not restricted to DCs, but also expressed by other immune cells, such as macrophages in the alveoli (Gonzalez-Juarrero et al., 2003) and adipose tissues (Brake et al., 2006). In humans, both monocytes and dendritic cells express CD11c (Collin et al., 2012).
What are dendritic cells?
Dendritic cells (DCs) are professional antigen-presenting cells that link innate and adaptive immunity and are critical for the induction of protective immune responses against pathogens.
What is Ly6G?
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow.
Do dendritic cells express cd20?
Plasmacytoid dendritic cells (pDCs) are known mainly for their secretion of type I IFN upon viral encounter. These CD2hiCD5+CD81+ cells express classical pDC markers, as well as the toll-like receptors that enable conventional pDCs to respond to viral infection.
Where are dendritic cells?
Dendritic cells are found in tissue that has contact with the outside environment such as the over the skin (present as Langerhans cells) and in the linings of the nose, lungs, stomach and intestines. Immature forms are also found in the blood.
Are PDC myeloid cells?
Plasmacytoid dendritic cells (pDCs) can be derived from both myeloid and lymphoid progenitors.
What does CD11c do in dendritic cells?
CD11c, also known as integrin alpha X, is the most widely used defining marker for dendritic cells (DCs). CD11c can bind complement iC3b and mediate phagocytosis in vitro, for which it is also referred to as complement receptor 4. However, the functions of this prominent marker protein in DCs, especially in vivo, remain poorly defined.
Is the mouse dendritic cell marker CD11c downregulated upon cell activation?
The mouse dendritic cell marker CD11c is down-regulated upon cell activation through Toll-like receptor triggering Dendritic cells (DC) play a key role in regulating immune responses and are the best professional antigen-presenting cells. Two major DC populations are defined in part according to cell surface CD11c expression levels.
What is the best marker for dendritic cells?
7 Howard Hughes Medical Institute, University of California, San Francisco, CA 94143. CD11c, also known as integrin alpha X, is the most widely used defining marker for dendritic cells (DCs). CD11c can bind complement iC3b and mediate phagocytosis in vitro, for which it is also referred to as complement receptor 4.
What are the hematopoietic markers of dcdcs?
DCs constitutively express the hematopoietic surface markers CD45, MHC class II, Flt3, and CD11c, and lack T cell, natural killer (NK) cell, B cell, granulocyte, and erythrocyte lineage markers (PMID: 23516985 ). In mice, CD11c is a well-established, yet not exclusive DC marker.