How does a hollow fiber bioreactor work?

How does a hollow fiber bioreactor work?

Modeled after the mammalian circulatory system, hollow fiber bioreactors offer the most in-vivo like manner to grow cells in any laboratory. These fibers then create a semi-permeable barrier of defined molecular weight cut-off (MWCO) between the compartment in which the cells are growing and the medium is flowing.

How does a hollow fiber membrane work?

Inside a hollow fiber membrane water filter, hundreds of tubes—resembling tiny straws—are bundled together to create a filter matrix. That is, the pores in the fibers’ walls are simply too small for the pathogens to fit through, but water still can. These pores so microscopic, they’re invisible to the naked eye.

What is a benefit of hollow fibers?

There are several advantages of the hollow fiber bioreactor system that allow for enhanced cell growth. Perhaps most prominent is that cells are allowed to grow in 3D culture at very high densities, which provides more in vivo-like conditions and healthier cells.

What is a bioreactor PDF?

Bioreactor is a vessel in which a chemical process is carried out which involves organisms (mainly microbes-viruses or bacteria, fungi and yeasts –traditionally designated as „Fermenters“) or biochemically active substances (enzymes, e.g.) derived from such organisms –in opposite to fermenters frequently considered as …

What is a perfusion bioreactor?

Our perfusion bioreactor systems allow for continuous feeding of the cell culture chamber from an external media bottle. While the culture media continuously perfuses through the system, the 3D aggregates of cells and scaffolds are retained in the cell culture chamber by a porous perfusion core.

What are some characteristics of hollow fibers?

The hollow fiber usually has a membrane coated on the outside of a porous fiber support as shown in Fig. 4.9. Hollow fiber modules are characteristically 4–8 in. (10–20 cm) in diameter and 3–5 ft (1.0–1.6 m) long.

Where are hollow fiber membranes used?

Hollow fiber membrane modules are used for a wide range of applications including dialysis [1], filtration [2], contactors [3], and gas separations [4]. Typically, the modules consist of a bundle of randomly packed hollow fibers.

How is a bioreactor designed?

Bioreactor design is a relatively complex engineering task, which is studied in the discipline of biochemical/bioprocess engineering. Nutrients may be continuously added to the fermenter, as in a fed-batch system, or may be charged into the reactor at the beginning of fermentation.

What is tower bioreactor?

This article considers the applicability of tower bioreactors without mechanical agitation (bubble columns) for cell mass production on alcohol and glucose substrates. These bioreactors are especially attractive because of their high oxygen transfer rate and low energy requirement.

What is ATF perfusion?

Source: Refine Technology, LLC. The ATF™ System was originally designed for perfusion processes in mammalian cell culture, using hollow fiber filters to achieve an efficient cell separation with low shear and allowing robust large scale manufacturing.

What is the difference between fed-batch and perfusion?

The fed-batch method adds nutrients once they are depleted. The perfusion method circulates medium through a growing culture, allowing simultaneous removal of waste, supply of nutrients, and harvesting of product.

What is a hollow fiber bioreactor?

A Hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which are small, semi-permeable capillary membranes arranged in parallel array with a typical molecular weight cut-off (MWCO) range of 10-30 kDa.

What is a hollow fiber membrane?

A hollow fiber membrane is a tubular, self-supporting membrane with a fiber diameter of less than 500 μm [73]. These membranes are prepared by phase inversion in a hollow fiber spinning setup.

How do hollow fiber membranes enhance gas–liquid mass transfer?

Hollow fiber membranes (HFMs) can effectively enhance the gas–liquid mass transfer in aqueous. In this type of reactor, syngas is diffused through micro-size pores of membrane without forming bubbles. The microbial community grows as a biofilm formed on the outer wall of the membranes ( Munasinghe and Khanal, 2010 ).

What is hollow fiber membrane perfusion in cell culture media?

Cell culture medium is pumped through the IC space and delivers oxygen and nutrients to the cells via hollow fiber membrane perfusion. As the cells expand, their waste products and CO 2 also perfuse the hollow fiber membranes and are carried away by the pumping of medium through the IC space.

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