What is KF water factor?
How do you calculate Karl Fischer factor? The water equivalence factor F is determined according to the formula 0.1566 x w / v in mgs of H2O per ml of reagent, where W is the sodium tartrate weight in mgs, and V is the reagent volume in ml.
What is KF technique?
Karl Fischer titration is a widely used analytical method for quantifying water content in a variety of products. The fundamental principle behind it is based on the Bunsen Reaction between iodine and sulfur dioxide in an aqueous medium.
What is the limit of KF factor?
Standardization is valid, if concentration should be not less than 5 mg and not more than 7mg. The relative Standard deviation between two average KF factors of water and DST determined consecutively should not be more than 3.0 % [Average of two factors (2 with water and 2 with DST) to be considered for calculation.]
What is difference between KF and Lod?
The Important difference between KF and LOD is, KF determines only moisture content in the sample and LOD determines weight loss of sample due to sample drying including volatile impurities.
What is KF test?
Karl Fischer (KF) Titration is a technique for the determination of moisture content. It is a process based on the reaction of iodine with water. The KF method is one of the few techniques that will measure water content and not be affected by other volatiles.
What is KF in pharma?
Karl Fischer reagent method of water content determination is widely used in pharmaceuticals and other manufacturing industries. This is most reliable and accurate method for moisture determination. The method to use is the Karl Fischer (KF) moisture analysis.
What is KF reagent?
As shown in Formula (1) below, the Karl Fischer method uses Karl Fischer reagent, which reacts quantitatively and selectively with water, to measure moisture content. Karl Fischer reagent consists of iodine, sulfur dioxide, a base and a solvent, such as alcohol.
Why methanol is used in KF titration?
Most liquid samples dissolve in methanol, which is the usual solvent in Karl Fischer determinations. To determine the total amount of water, the sample must dissolve completely. Other solvents can be added to methanol in specific proportions to liberate the water more efficiently.
What is LOD and LoQ in pharma?
LoD is the lowest analyte concentration likely to be reliably distinguished from the LoB and at which detection is feasible. LoQ is the lowest concentration at which the analyte can not only be reliably detected but at which some predefined goals for bias and imprecision are met.
How do you make KF reagent?
Preparation of the KF Reagent Prepare a solution by mixing 670 mL of methanol and 170 mL of pyridine. Now, add 125 g of iodine to the solution and cool it. Then, take a 250 mL graduated cylinder and add 100 mL of pyridine to it. Keep the solution in an ice bath.
What electrode is KF titration?
The most advanced KF titration technology uses a double platinum electrode for electrochemical indication of the end point, but visual and photometric indicators are also used.
Why is DST used in KF calibration?
Sodium tartrate dihydrate is the volumetric standard for Karl Fischer titration. Under normal conditions, it is stable and non-hygroscopic. Sodium tartrate dihydrate has a stoichiometric water content of 15.66% and is primarily used for titer determination in volumetry.
What is KF titration?
Published a method in 1935 to determine trace amounts of water in samples. This method is now called Karl Fischer titration. Abbreviations: KF or KFT It remains the primary method of water content determination used worldwide by: Government – Food Science
How do you determine the KF of water?
The vial is placed in the oven chamber and heated, a carrier gas transfers the released water to the titration cell, where it is then determined by KF titration. Depending on the sample’s water content, the subsequent KF water determination can be carried out volumetrically or, for trace amounts, coulometrically.
What is the Karl Fischer test for moisture?
The Karl Fischer Method of Moisture Detection Applied Technical Services performs Karl Fischer testing to determine the moisture content of sample materials. We maintain a state of the art Karl Fischer unit capable of volumetric and coulometric titration, which offers a detection limit of 5ppm ±5 µg.
What is the accuracy of KF analysis?
KF has a high accuracy and precision, typically within 1% of available water, e.g. 3.00% appears as 2.97–3.03%. Although KF is a destructive analysis, the sample quantity is small and is typically limited by the accuracy of weighing.
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