What is KeratinoSens?
View details. The KeratinoSens is a reporter gene assay which uses an immortalised adherent cell line derived from an expanded clone of HaCaT human keratinocytes.
Is Nrf2 a luciferase test?
The ARE-Nrf2 Luciferase Test is an in vitro test method that models the second key event in the skin sensitization adverse outcome pathway.
What is a luciferase assay?
A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene. Luciferase is an enzyme used for bioluminescence by various organisms in nature, most famously the firefly.
What’s the meaning of luciferin?
Definition of luciferin : any of various organic substances in luminescent organisms (such as fireflies) that upon oxidation produce a virtually heatless light.
What is the purpose of luciferase?
The power of luciferase has been harnessed by scientists to devise reactions whose light output is used to monitor biological processes including gene expression, biomolecular binding, and cell viability.
Why is luciferase important?
What is the role of luciferase?
Luciferases are proteins with enzymatic activity that, in the presence of ATP, oxygen, and the appropriate substrate (typically luciferin), catalyze the oxidation of the substrate in a reaction that results in the emission of a photon.
What is luciferase RNA?
Luciferase Control RNA is an uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail that produces functional luciferase when translated. Control reactions are monitored easily by a luciferase assay for the production of luminescence generated from the full-length luciferase.
How is luciferase produced?
Luciferases can be produced in the lab through genetic engineering for a number of purposes. Luciferase genes can be synthesized and inserted into organisms or transfected into cells. As of 2002, mice, silkworms, and potatoes are just a few of the organisms that have already been engineered to produce the protein.
How is luciferase seen?
Luciferase-labeled islets emit visible light detected by bioluminescence imaging. While this technique is very sensitive, it is dependent on light attenuation through tissue before reaching the detector and the sensitivity of the charge-coupled device that captures light emission.
What does the word luciferase mean?
Definition of luciferase : an enzyme that catalyzes the oxidation of luciferin.