How does jc1 work?
JC-1 as a fluorescent cationic carbocyanine dye exhibits potential-dependent accumulation in mitochondria, which form J aggregates and it diffuses across mitochondria to form monomeric state upon depolarization (Figure 1).
What is a JC-1 assay?
The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry. View a selection guide for all apoptosis assays for flow cytometry. Selective to mitochondrial changes. The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health …
What is mitochondrial membrane potential assay?
The Mitochondrial Membrane Potential Assay Kit (II) is a fluorescent assay that detects the mitochondrial membrane potential in living cells.
Who discovered Mitophagy?
Mitophagy is the selective degradation of mitochondria by autophagy. It often occurs to defective mitochondria following damage or stress. The process of mitophagy was first described over a hundred years ago by Margaret Reed Lewis and Warren Harmon Lewis.
What is mitochondrial depolarisation?
When a mitochondrion depolarizes, it stops producing ATP, and as more mitochondria depolarize in the cell due to the increased mPTP open probability, the cytosolic ATP decreases. Since ATP is required for the SERCA pump, a low ATP level could impair the SERCA activity to promote Ca2+ alternans.
Can you fix MitoTracker?
For MitoTracker® Red CMXRos, we have found that fixing with 3.7% formaldehyde in complete growth medium at 37°C for 15 minutes works well for endothelial cells.
How long does MitoTracker last?
approximately 48 hours
The experiment will last approximately 48 hours.
How do you test mitochondrial membrane potential?
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells.
What triggers mitophagy?
► Mitophagy is specifically induced by mild and transient oxidative stress. ► Moderate levels of reactive oxygen species do not trigger non-selective autophagy. ► ROS and starvation induced mitophagy occurs in a DRP1-dependent manner. ► Starvation induced hyperfusion of mitochondria counteracts mitophagy.
Is mitophagy an autophagy?
Overview. Mitophagy is a process often regulated independently of the nutrient/energy/stress signals that govern basal autophagy (7). It operates primarily as a quality control mechanism, targeting dysfunctional mitochondria that may otherwise contribute to the activation of death signaling (7).
Can mitochondria divide independently?
Mitochondria are unusual organelles. They also divide independently of the cell in which they reside, meaning mitochondrial replication is not coupled to cell division.