What is a hybridization probe used for?

What is a hybridization probe used for?

In molecular biology, a hybridization probe (HP) is a fragment of DNA or RNA of usually 15–10000 nucleotide long which can be radioactively or fluorescently labeled. HP can be used to detect the presence of nucleotide sequences in analyzed RNA or DNA that are complementary to the sequence in the probe.

What is the best method for detection of Chlamydia trachomatis?

Nucleic acid amplification tests are the most sensitive tests for detecting chlamydia infection, and may be performed on endocervical, urethral, vaginal, pharyngeal, rectal, or urine samples.

What is chlamydia amplified probe?

The Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) uses transcription-mediated amplification and hybridization protection assay procedures to qualitatively detect Chlamydia trachomatis rRNA in urine, endocervical swab, and urethral specimens.

Can chlamydia be diagnosed by PCR?

These tests are very good at identifying chlamydia. A test that is positive almost always means the problem is there and is unlikely to be a false-positive test result. A polymerase chain reaction (PCR) test is an example of a nucleic acid amplification test. This test can also be done on a urine sample.

How does DNA hybridization work?

Hybridization of DNA is accomplished by heating strands of DNA from two different species to 86° C [186.8° F]. This breaks the hydrogen bonds between all complementary base pairs. The result is many single-stranded segments of DNA. The single-stranded DNA from both species is mixed together and allowed to slowly cool.

What is a chlamydia DNA probe test?

Nucleic acid amplification tests (NAAT) are used to find chlamydia infection. These tests use urine or a sample of body fluid from areas such as the cervix, vagina, eyes, rectum, or throat to find the genetic material (DNA) of chlamydia bacteria. These tests are very good at identifying chlamydia.

What does chlamydia DNA detection mean?

Test Overview Nucleic acid amplification tests (NAAT) are used to find chlamydia infection. These tests use urine or a sample of body fluid from areas such as the cervix, vagina, eyes, rectum, or throat to find the genetic material (DNA) of chlamydia bacteria. These tests are very good at identifying chlamydia.

What is GC amplified DNA?

The CT and GC Direct Amplified Test is a gene amplification based technology that represents one of the most sensitive methods for the detection of CT and GC in urogenital samples. This test has the important added advantage that patient urine samples may be used to reliably screen for the presence of CT and GC.

What is chlamydia DNA PCR?

Chlamydia trachomatis is the most common mandatory reporting STD (STI) in Quebec. It is often asymptomatic and, if left untreated, can lead to complications affecting the genital organs and fertility. The screening technique (PCR or NAAT) detects the bacteria’s DNA or RNA (genetic material) after amplification.

What is DNA hybridization in microbiology?

Hybridization is the process of combining two complementary single-stranded DNA or RNA molecules and allowing them to form a single double-stranded molecule through base pairing.