What are spectrophotometers used for?
Spectrophotometry applications are useful to measure the absorbance, reflectance, and transmission of light by gases, liquids, and solids.
What are the types of spectrophotometers?
The spectrophotometer can be divided into five subcategories according to the wavelength and application context :
- VIS spectrophotometer.
- UV-VIS spectrophotometer.
- Infrared spectrophotometer.
- Fluorescence spectrophotometer.
- Atomic absorption spectrophotometer.
What is a spectrophotometer for dummies?
A spectrophotometer is an analytical instrument that finds use to quantitatively measure the transmission or reflection of visible light, UV light, or infrared light. Spectrophotometers measure the intensity of electromagnetic energy in a specified region as a function of light source wavelength.
What does the Beer-Lambert law state?
Beer’s law (sometimes called the Beer-Lambert law) states that the absorbance is proportional to the path length, b, through the sample and the concentration of the absorbing species, c: A α b · c. The proportionality constant is sometimes given the symbol a, giving Beer’s law an alphabetic look: A = a · b · c.
What is the principle of UV VIS spectroscopy?
The Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, which results in the production of distinct spectra. Spectroscopy is based on the interaction between light and matter.
What is Beer’s law used for?
Beer’s Law is used in chemistry to measure the concentration of chemical solutions, to analyze oxidation, and to measure polymer degradation. The law also describes the attenuation of radiation through the Earth’s atmosphere.
What are two basic types of spectrophotometers?
There are two major classes of devices: single-beam and double-beam. A double-beam spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample.
How is beer Lambert law used in spectroscopy?
The Beer-Lambert law states that there is a linear relationship between the concentration and the absorbance of the solution, which enables the concentration of a solution to be calculated by measuring its absorbance.
What is absorbance and transmittance?
Absorbance (A), also known as optical density (OD), is the quantity of light absorbed by a solution. Transmittance is the quantity of light that passes through a solution.