What are HEp-2 cell patterns?
When evaluating cell staining results for HEp-2, five major nuclear patterns are most commonly reported: homogenous, speckled, centromere, nucleolar, and nuclear dots. These patterns are the result of autoantibody binding to specific constituents of the nucleus.
What does a positive ANA HEp-2 mean?
A positive result on an ANA test means that antinuclear antibodies were found in your blood. You may get a positive result if: You have SLE (lupus). You have a different type of autoimmune disease. You have a viral infection.
What is a HEp-2 slide?
MBL Bion ANA (HEp-2) ANTIGEN SUBSTRATE SLIDES are intended for use as an aid in the diagnosis of certain autoimmune diseases. Among the methodologies available to detect ANA’s are EIA, ELISA, Dot Blot and the Indirect Fluorescent Antibody (IFA) technique.
What is ANA by HEp-2 cells?
Summary: The presence of antinuclear antibodies (ANA) indicates the possibility of autoimmunity and the indirect immunofluorescence (IIF) assay on HEp-2 cells is the standard blood test (ANA-HEp-2) used to detect ANA.
Where are HEp-2 cells from?
The HEp-2 cell line originated from tumours produced after injection of rats with epidermoid carcinoma tissue.
Where do HEp-2 cells come from?
How do you read ANA results?
Your test is positive if it finds antinuclear antibodies in your blood. A negative result means it found none. A positive test doesn’t mean that you have an autoimmune condition. Between 3% and 15% of people with no conditions have antinuclear antibodies.
What is used as the substrate for ANA testing?
In the early days of ANA testing, rodent tissue (stomach, liver and/or kidney) was commonly used as the substrate. Rodent tissue however had several drawbacks such as small cell size, a lack of dividing cells (mitotics) and poor antigen expression that made interpretation of ANA patterns difficult.
What are hep cells?
Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.