Which is the function of polynucleotide kinase?
Polynucleotide kinase/phosphatase (PNKP) processes strand-break termini to render them chemically compatible for the subsequent action of DNA polymerases and ligases.
Which of the following enzymes is involved in base excision repair?
DNA glycosylase
DNA glycosylase is an enzyme involved in the base excision repair.
Which type of excision repair takes care of minor DNA damage?
Base excision repair (BER) corrects DNA damage from oxidation, deamination and alkylation. Such base lesions cause little distortion to the DNA helix structure.
When was base excision repair discovered?
The discovery of nucleotide excision repair in 1964 showed that DNA could be repaired by a mechanism that removed the damaged section of a strand and replaced it accurately by using the remaining intact strand as the template. This result showed that DNA could be actively metabolized in a process that had no precedent.
Does base excision repair occur in prokaryotes?
Introduction. The major pathway for the removal of oxidative base damage is the DNA base excision repair pathway, found in prokaryotes and eukaryotes (1).
Which molecules are common between base excision repair and nucleotide excision repair?
Whereas RER and MMR from last time repair replication errors, base excision repair (BER) and nucleotide excision repair (NER) repair chemical damage….Molecular Biology 12: ‘Base excision repair (BER) and nucleotide excision repair (NER)’
| BER | NER | |
|---|---|---|
| repairs damage caused by | endogenous mutagens | exogenous mutagens |
Which of the following are involved in base excision repair of DNA?
BER requires only four or five enzymes in the basic reaction steps to carry out repair of DNA containing AP sites or base damage. These include a DNA glycosylase, an AP endonuclease, a DNA polymerase, and a DNA ligase 15. BER is initiated with excision of a damaged base by the DNA glycosylase.
Which components are part of base excision repair?
Proteins involved in base excision repair
- DNA glycosylases.
- AP endonucleases.
- End processing enzymes.
- DNA polymerases.
- Flap endonuclease.
- DNA ligase.
- MBD4.
- NEIL1.
What is the role of T4 polynucleotide kinase in gene cloning Mcq?
Catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates.
How many polypeptide chains are present in active insulin Mcq?
two polypeptide chains
Active insulin consists of how many polypeptide chains? Explanation: Active insulin consists of two polypeptide chains, the A chain and the B chain. The A chain consists of 21 amino acids and the B chain consists of 30 amino acids.