What is the isoelectric point of bovine serum albumin?
The isoelectric point of BSA is at pH 4.5. At this pH, the net surface charge becomes zero and BSA molecules aggregate.
What is isoelectric pH of albumin?
During the N–F isoform transition, bovine serum albumin (BSA) passes through its isoelectric point at pH 4.7 and the net charge on the protein changes from −16 at pH 7.4 to +100 at pH 3.5.
What is the pH of albumin?
Albumin is a globular, water-soluble, un-glycosylated serum protein of approximate molecular weight of 65,000 Daltons. Albumin (when ionized in water at pH 7.4, as found in the body) is negatively charged.
What color is bovine serum albumin?
BOVINE SERUM ALBUMIN 30% Solution contains a 30% solution of bovine serum albumin (Fraction V, Immunoglobulin and Protease Free) in saline with preservative and stabilizer. The product is ~30% protein as determined by Biuret assay. A clear yellow green to dark amber color is typical.
What is isoelectric point?
The isoelectric point (pI) is the pH of a solution at which the net charge of a protein becomes zero. At solution pH that is above the pI, the surface of the protein is predominantly negatively charged, and therefore like-charged molecules will exhibit repulsive forces.
What is BSA used for?
Bovine Serum Albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other serum components are unwanted. In cell culture, it acts as a small molecule carrier.
Is albumin acidic or basic?
Albumin is a major drug-binding protein in serum. Albumin is an alkaline protein, so acidic and neutral drugs primarily bind to it.
Is Bovine Serum Albumin glycosylated?
BSA: bovine serum albumin; BSA–DT10 and BSA–DT20 are referred to glycosylated BSA by conjugation with dextran (10 kDa and 20 kDa) via Maillard reaction.
What is the function of bovine serum albumin?
Bovine Serum Albumin (BSA) is used in a variety of laboratory applications including its function as a protein concentration standard, its function as a cell nutrient and its ability to stabilize enzymes during restriction digest.
Why is BSA used?
BSA is used because of its ability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.
What is isoelectric pH and its significance?
The isoelectric point (pI) is the pH at which a particular molecule carries no net electrical charge. The net charge on the molecule is affected by the pH of its surrounding environment and can become more positive or negative due to the gain or loss of protons, respectively.
What is meant by isoelectric pH of an amino acid?
The isoelectric point of an amino acid is the point at which the amino acid has no net electrical charge.