What is the excitation and emission wavelength for FITC?

What is the excitation and emission wavelength for FITC?

FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.

What is the best excitation light for FITC?

A fluorophore’s maximal absorbance informs you which laser line is optimal to be used for excitation. In the case of FITC, its maximum absorbance falls within the blue spectrum. Therefore, the blue 488 nm laser, which is close to FITC’s absorbance peak of 490 nm, is commonly used to excite this fluorophore.

Is FITC a dye?

1 Fluorescein. Fluorescein isothiocyanate (FITC) is the most widely used fluorescent probe for the preparation of conjugates with biological molecules (Hansen, 1967; Haugland, 1990). This xanthene dye (Fig.

Are fluorescein and FITC the same?

FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all JIR antibodies and purified proteins, with the exception of streptavidin.

Is FITC same as 488?

Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. In addition, Alexa Flour 488 is pH insensitive (over a broad pH range) and tends to be brighter compared to FITC on most instruments.

What is FITC stain?

Fluorescein-5-isothiocyanate (FITC) is a fluorescence dye and belongs to the xanthene dyes. FITC is used for labeling of different biomolecules, e.g. immunoglobulins, lectins and other proteins, peptides, nucleic acids, nucleotides; oligo-and polysaccha- rides.

How do you excite GFP?

GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.

What does FITC stain?

FITC is used for labeling of different biomolecules, e.g. immunoglobulins, lectins and other proteins, peptides, nucleic acids, nucleotides; oligo-and polysaccha- rides. Tissue sections and cell monolayers may be stained in various ways with FITC.

Is FITC a 488?

The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.

Is af488 brighter than FITC?

In addition, Alexa Flour 488 is pH insensitive (over a broad pH range) and tends to be brighter compared to FITC on most instruments.

How stable is FITC?

FITC-dextran is stable in 6% trichloracetic acid at room temperature for 3 days2. Autoclaved FITC-dextran 70 solutions were stored at temperatures from 8 to 50 °C for periods up to 5 months.

What wavelength of light excites GFP?