What is L929 cell?
NCTC clone 929 [L cell, L-929, derivative of Strain L] (connective mouse tissue) is the first clone of strain L, one of the first cell strains to be established in continuous culture. Use NCTC clone 929 for cell line toxicity testing and as a transfection host.
How do you make a L929 conditioned medium?
Preparation of L929-Conditioned Medium
- Plate 4.7 × 105 L929 cells in a 75-cm2 flask containing 55 mL of L929 medium.
- Grow cells in a humidified incubator with 5% CO2 at 37°C for 7 d.
- Collect the supernatant. Filter through a 0.45-μm filter. Store 50-mL aliquots frozen at −20°C (L929-conditioned medium).
What type of cells are used in a continuous cell culture?
Continuous immortalized cell lines are comprised of a single cell type that can be serially propagated in culture either for a limited number of cell divisions (approximately thirty) or otherwise indefinitely. Cell lines of a finite life are usually diploid and maintain some degree of differentiation.
What are peritoneal macrophages?
Peritoneal macrophages are the macrophages that reside in the peritoneal cavity, a fluid-filled space located between the wall of the abdomen and the organs found in the abdomen. In the absence of peritoneal infection or inflammation, peritoneal macrophages are thought to have anti-inflammatory functions.
What is complete medium?
A culture medium that is enriched to contain all of the growth requirements of a strain of organisms.
Are CHO cells immortal?
Fact #3 – CHO cells are not immortal Well, while they are tiny yet tough, they are not “immortal”. Much rather, they are what is called “immortalized”. The name stems from the cells’ apparent ability to be cultured indefinitely.
What is the origin of L929 cells?
L929 is a fibroblast cell line derived from a clone of normal subcutaneous areolar and adipose tissue of a male C3H/An mouse (9, 10). It was found to secrete a macrophage growth factor (11), which was later identified as M-CSF (4).
What is the composition of L929 cell-conditioned media in bmdms?
BMDMs are a key model system to study macrophage biology in vitro. Commonly used methods to differentiate macrophages from BM are treatment with either recombinant M-CSF or the supernatant of L929 cells, which secrete M-CSF. However, little is known about the composition of L929 cell-conditioned media (LCCM) and how it affects the BMDM phenotype.
Can L929 cell conditioned media protect mouse peritoneal macrophages from oxidative injury?
1 Research Laboratory of Free Radical Medicine, The First Military Medical University, Guangzhou, People’s Republic of China. [email protected] We have previously found that L929 cell conditioned medium (L929-CM) could protect mouse peritoneal macrophages from oxidative injury.
How are L929 cells maintained in DMEM?
L 929 cells are maintained in T-75 cm2 filter cap flasks in DMEM ( Invitogen, Canada #11960-069) + 1% Penicillin Streptomycin / L-glutamine (Invitogen, Canada #10378-016) + 10% heat inactivated Fetal Bovine Serum (56°C,30 min.) and 7.5% CO2. The Fetal Bovine Serum is the same as used for cultivation of the Bone Marrow Derived Macrophages.