What is Isoton diluent?
Beckman Coulter™ ISOTON™ II Diluent is a filtered, phosphate-buffered saline solution compatible with human blood cells, and may be used for the suspension of most biological cells and many industrial samples. Dilution Agent. For use with Flow Cytometers. pH 7.35-7.65.
What is Isoton II?
Isoton II is a balanced electrolyte solution which may be used as a diluent for cell counting and for general purpose use. Bacteriostatic and Fungistatic. Has a typical background count of <50 counts/ml when using a 100 micron aperture tube.
What is Beckman Coulter method?
The Coulter Principle is based on the detection and measurement of changes in electrical resistance produced by a particle or cell suspended in a conductive liquid (diluent) traversing through a small aperture. When particles or cells are suspended in a conductive liquid, they function as discrete insulators.
What is the principle of Coulter Counter?
The Coulter counter uses the principle that the electrical resistance of a conducting liquid is increased by the addition of an insulating material. Particles are assessed individually. To obtain adequate sensitivity, the volume of liquid measured must be similar to the volume of the particle.
What instrument is used for CBC?
The CBC is performed using basic laboratory equipment or an automated hematology analyzer, which counts cells and collects information on their size and structure.
How does particle size affect solubility?
The disjoining pressure of small particles is greater than that of large particles, so small particles have a higher interfacial solubility. Due to their higher differential concentration, thinner diffusion layer,27 and increased surface area, small particles dissolve faster (Figure 8A).
What are hematology reagents?
Hematology reagents are the substances or chemicals intended to perform qualitative and/or quantitative analyses of the morphology of the blood, its components, and blood-forming tissues. These reagents include those used for blood cell counts, coagulation testing, other hematology tests, control, and calibration.
Which test is used in Coulter counter method?
Red blood cell counts (RBC), mean corpuscular volume (MCV) of red blood cells, and platelet counts may be determined using an automated device such as a Coulter counter to take direct measurements.
How does size relate to solubility?
Compounds with small ions are less soluble than compounds with large ions. Small ions are closer to each other, so they have strong attractive forces. It is more difficult for the water to break them apart, so they are less soluble. The solubility increases as the size of the halide ion increases.
What are 4 factors that affect solubility?
There are four major factors that affect the solubility of gases inside liquids. Pressure, temperature, size and chemical reactivity between the liquid and gas. By increasing the pressure, we usually increase the solubility of the gas inside the liquid as per Henry’s law.