What is immunophenotyping in AML?

What is immunophenotyping in AML?

Immunophenotyping by flow cytometry of bone marrow or peripheral blood samples can be used to help distinguish AML from acute lymphocytic leukemia (ALL) and further classify the subtype of AML.

What is immunophenotyping in leukemia?

Immunophenotyping is a test used to identify cells on the basis of the types of markers or antigens present on the cell’s surface, nucleus, or cytoplasm. This technique helps identify the lineage of cells using antibodies that detect markers or antigens on the cells, hence the “immuno-” prefix.

What are the markers for AML?

By flow cytometry, the blasts in acute myeloid leukemia (AML) with myelodysplasia-related changes (AML-MRC) show a myeloblast phenotype, with expression of blast markers (CD34, CD117) and myeloid markers such as CD13, CD33, and/or myeloperoxidase (MPO); aberrant expression of CD5, CD7, or CD56 may also be seen.

What is immunophenotyping used for?

Immunophenotyping is used primarily to help diagnose and classify the blood cell cancers, leukemias and lymphomas, and to help guide their treatment.

Is immunophenotyping same as flow cytometry?

The most common use of flow cytometry is in the identification of markers on cells, particularly the immune system or immunophenotyping. It is the desire to detect an increased number of cell subsets of the immune system that has driven the increase in multicolor flow cytometry.

What is non M3 AML?

Hypergranular non-M3 AML is difficult to diagnose by FC by pattern recognition because the blasts overlap with maturing granulocytes by SSC/CD45 gating criteria, and are not located in the blast gate. In this respect, they mimic APL and regenerative marrows, including those following G-CSF therapy.

How immunophenotyping test is done?

Immunophenotyping is a test in which the cells in a sample of blood or bone marrow are looked at under a microscope to find out if malignant lymphocytes (cancer) began from the B lymphocytes or the T lymphocytes. The process identifies cells based on the types of antigens or markers on the surface of the cell.

What is flow cytometry immunophenotyping?

Immunophenotyping by flow cytometry is a laboratory method that detects the presence or absence of white blood cell (WBC) markers called antigens. These antigens are protein structures found on or within WBCs. Additionally, specific patterns of antigens are present on abnormal cells seen in leukemias and lymphomas.