How do you convert kDa to molecular weight?
1 Dalton (Da) = 1 g/mol, this means that 1 KDa = 1000 g/mol = 1 kg/mol.
How do you convert ug mL to nM?
The simple formula is: ( µg/mL ) = ( µM ) * ( MW in KD) , ( ng/mL ) = ( nM ) * ( MW in KD) , ( pg/mL ) = ( pM ) * ( MW in KD) . For example: If the protein molar concentration is labeled as 2 µM, and the MW of the protein is 40 KD, then this protein product’s mass concentration will be 2 ( µM ) * 40 ( KD ) = 80 µg/mL.
What is kDa in molecular weight?
Dalton (Da) is an alternate name for the atomic mass unit, and kilodalton (kDa) is 1,000 daltons. Thus a peptide with a mass of 64kDa has a molecular weight of 64,000 grams per mole.
How big is a protein in nM?
A simple rule of thumb for thinking about typical soluble proteins like the Rubisco monomer is that they are 3-6 nm in diameter as illustrated in Figure 1 which shows not only Rubisco, but many other important proteins that make cells work.
How many AA are in a kDa?
Protein of molecular weight of 6.7kDa contain approximately 609.90 amino acids.. Bcz we know that approximate weight of one amino acids is 110 so divide it by 110 we can get this number.
Is g mol same as kDa?
Measure of molecular weight or molecular mass. One molecular hydrogen molecular atom has molecular mass of 1 Da, so 1 Da = 1 g/mol. Proteins and other molecular macromolecule molecular weights are usually measured in molecular kDa or kD (kilodaltons) – 1000 Da.
How do I make a 50 ug mL solution?
Yes make the units the same. So convert 100 mg/mL into ug/mL = 100,000 ug/mL. You will now see that your stock requires a 10000x dilution to reach 10 ug/mL and will require a 2000x dilution to reach 50 ug/mL. You can use c1v1=c2v2 to determine the volumes required to perform the dilutions.
Is KD a Kilodalton?
kD may also refer to the symbol used for unit, kilodalton (also, kDa). One kilodalton is equivalent to 1,000 daltons. Dalton is a measure of the weight of a hydrogen atom. Kawasaki disease, for instance, is abbreviated as KD.
How is Kilodalton measured?
Measure of molecular weight or molecular mass. One molecular hydrogen molecular atom has molecular mass of 1 Da, so 1 Da = 1 g/mol. Proteins and other molecular macromolecule molecular weights are usually measured in molecular kDa or kD (kilodaltons) – 1000 Da. molecular average molecular amino molecular acid = 110 Da.
How do you calculate protein size?
All Answers (9) Whatever program you use to visualize protein structures should have tool to measure the distance between any 2 atoms in the structure. Find the 2 atoms that appear to be the farthest apart, and measure the distance between them. That is the size of the protein in that dimension.
What is the normal size of amino acid in NM?
Explanation: we know that amino acids are nothing but single structure monomers of proteins. thus they are visible only under an ultra microscope due to is an extreme small size in the range of nano meters. their size ranges from 1 to 2 nm.